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Growth Autotransplantation

Autotransplante de peroné con cartílago de crecimiento Tibialización del peroné en osteosarcoma de Tibia

Tibial Growth Plate Autotransplantation

Summary

  Tibia autotransplantation. Bone injuries, which occur in the proximal region of the tibia in children, can affect the growth physis and represent a challenge for orthopedic treatment to reconstruct the bone defect created. Reconstruction methods do not always consider the bone growth potential of this segment. The objective of this work is to present a new surgical technique for bone reconstruction, based on the transposition of the ipsilateral fibula, with its growth physis, without the need for a microsurgical technique and using a sliding internal fixation device. Material and Method: The authors report two cases of patients with bone sarcoma in the proximal region of the tibia, with involvement of the growth cartilage, which were treated using the proposed technique. Results: In both cases there was bone consolidation, hypertrophy and longitudinal growth of the transposed fibula. Conclusion: The technique, proposed by the authors, maintained the vascularization of the auto-transplanted bone segment, without the need for microsurgery and preserved the potential for physeal growth. The implant used allowed longitudinal growth of the bone, evidenced radiographically. Level of evidence IV. Case series.

   Descriptors: growth, sliding, device, fibula, physis, fixation, tibialization sarcoma, transplant.

Abstract

   Bone lesions, which occur in the proximal tibia in children, can affect the growth plate and represent a challenge to the orthopedic treatment for reconstruction of bone defects created. Reconstruction methods do not always compensate the potential for bone growth in this segment. The objective of this paper is to present a new surgical technique of bone reconstruction, based on the transposition of the ipsilateral fibula with it’s growth plate, without the need for microsurgical technique and using a sliding internal fixation device. Material and Method: The authors report two cases of patients with bone sarcoma at the proximal tibia, affecting the growth cartilage, which were treated by the proposed technique. Results: In both cases, there were bone healing, hypertrophy and longitudinal growth of the transposed fibula. Conclusion: The technique proposed by the authors, kept the vascularization of bone segment auto-transplanted without the need for microsurgery and preserves physeal growth potential. The implant used allowed the longitudinal bone growth, as radiographically seen.  Levels of evidence IV. Case series.

Keywords: growth, sliding, device, fibula, physis, fixation, sarcoma, tibialisation, transplant.

INTRODUCTION

   The proximal third of the tibia, in the skeletally immature population, houses a growth physis responsible for approximately 30% of the final length of the limb in adulthood 1 . This region is also the second most common location for the incidence of primary bone tumors, behind only the distal third of the femur 2 .

   Tumors that arise in the proximal region of the tibia, before skeletal maturity, can affect the growth physis and generate discrepancies in the final length of the lower limbs. The same can occur after trauma or secondary to diseases such as osteomyelitis, in this region.

   Tumor resection, in this segment of the tibia, requires reconstruction of the bone defect generated, which, due to young age, may have unsatisfactory results with traditional methods, recommending amputation as an alternative  (figures 1a, 1b and 1c).

Figura 1a: Região proximal da tíbia comprometida pelo tumor.
Figure 1a: Proximal region of the tibia affected by the tumor.
Figura 1b: esquema da falha a ser reconstruida.
Figure 1b: schematic of the fault to be reconstructed.
Figura 1c: segmento ressecado
Figure 1c: resected segment

Among the reconstructions of this segment, we can mention replacement with a non-conventional endoprosthesis, use of homologous or autologous grafts and bone transport 4-8 .

   None of the above methods replace the injured growth physis. Bone transport, which allows longitudinal stretching of the bone, may be unfeasible due to the need for prolonged use of an external fixator. In cancer patients, who are immunosuppressed by adjuvant chemotherapy, the fixator favors infections, in addition to requiring multiple surgeries to equalize the limbs in young children.

   The use of the vascularized fibula to fill bone defects gained great momentum with the development of the microsurgical technique, as it allows the use of the contralateral fibula 9-13 .

   The use of the proximal segment of the fibula with its physis, using a microsurgical technique, made it possible, for the first time, to reconstruct bone defects and reestablish the longitudinal growth of the bone 14 . However, it requires a specialized team, as well as special and high-cost materials and equipment, and is subject to several complications.

   The objective of this work is to present a new surgical technique for reconstructing bone injuries that compromise the proximal region of the tibia and its growth plate in children. This technique consists of transposing the ipsilateral fibula, together with its growth physis, preserving blood supply and the capacity for longitudinal growth, without the need for a microsurgical technique, using a single surgical access route. We describe two cases where this technique was used.

 

MATERIAL AND METHOD

 

   The medical records of two patients with bone sarcoma in the proximal region of the tibia, who presented growth cartilage impairment, were retrospectively analyzed and were treated surgically using the technique of autotransplantation of the proximal segment of the fibula to the tibia, developed by the co-author. Baptista*, in the Department of Orthopedics at Santa Casa de São Paulo.

 

Description of the surgical technique

 

   The patient is positioned in a horizontal supine position. A single, curved access route is used, starting above the proximal tibio-fibular joint, descending anteriorly along the tibial crest and curving medially, a few centimeters below the location where the fibular osteotomy will be performed (figure 2a) .

  The tibialis anterior muscle is exposed, the perimysium is opened and the muscle is moved laterally, leaving the inner layer of this perimysium adherent to the periosteum, aiming to preserve the oncological resection margin of the tibia (figure 2b).

 

 The neck of the fibula is identified and the common peroneal nerve isolated. The proximal tibiofibular joint is approached and the joint capsule, together with the anterior ligament, posterior ligament, arcuate popliteal ligament, fibular collateral ligament and the biceps femoris muscle tendon are released (figure 2c).

The proximal epiphysis of the tibia together with the anterior tuberosity are isolated from the metaphyseal region (figure 2d).

Figura 2a: Via de acesso única.
Figure 2a: Single access route.
Figura 2b: abertura do perimisio e lateralização do músculo tibial anterior.
Figure 2b: opening of the perimysium and lateralization of the tibialis anterior muscle.
Figura 2c: dissecção da porção proximal da fíbula.
Figure 2c: dissection of the proximal portion of the fibula.
Figura 2d: isolamento da epífise tibial.
Figure 2d: Isolation of the tibial epiphysis.

A Kirschner wire is passed through this epiphysis, horizontally, at the point where the proximal fixation will be made and the position of the plate is then verified (figure 3a).

The segment to be resected is measured, following the oncological margin, and the distal osteotomy of the tibia is performed, in the diaphyseal region. The posterior muscles of this portion are disinserted to the proximal epiphyseal region. The tibial epiphysis is then separated from the tumor by transepiphyseal osteotomy, preserving as much of the epiphyseal bone with its articular cartilage as possible and the tumor is resected (figures 3b and 3c).

Figura 3b: Separação da epífise tibial do tumor, por osteotomia trans-epifisária.
Figure 3a: Guide wire passing through the epiphysis. Figure 3b: Separation of the tibial epiphysis from the tumor, by trans-epiphyseal osteotomy.
Figura 3c: Tumor ressecado.
Figure 3c: Resected tumor.

To replace this bone defect, the proximal segment of the ipsilateral fibula is used, which is isolated from the tibiofibular joint and the lateral collateral ligament. A small deperiostization is performed, of one to two centimeters, at the height where the osteotomy will be made in the fibular shaft (figure 4a). After the osteotomy, this deperiostized part is nailed into the tibial diaphysis (figure 4b).

The proximal segment of the fibula, together with all the muscles and its nourishing arteries, is transferred to the center of the remaining tibial epiphysis and the fibular collateral ligament is inserted into the periosteum of the tibia (figure 4c).

Osteosynthesis with screws is performed using the Baptista extensible internal fixation device, placed on the medial side of the leg, which is previously custom-made for each case 15  (figure 4d).

Figura 4a: Pequena desperiostização do segmento distal da fíbula.
Figure 4a: Small deperiostization of the distal segment of the fibula.
Figura 4b: encavilhamento desta no canal medular da tíbia.
Figure 4b: nailing of this in the medullary canal of the tibia.
Figura 4c: posicionamento da fíbula sob o planalto tibial com reinserção do ligamento lateral.
Figure 4c: positioning of the fibula under the tibial plateau with reinsertion of the lateral ligament.
Figura 4d: osteossíntese proximal e distal com parafusos.
Figure 4d: proximal and distal osteosynthesis with screws.
This device is made up of two plates that are joined together by means of a trapezoidal-shaped rail, which fixes them and at the same time allows them to slide between them (figures 5a 1 and 5b 1 ). The proximal plate has a platform to support the remaining part of the tibial plateau and holes for its fixation to the epiphysis, carried out with special full-thread screws (figure 5a 2 ). The distal plate is made with a low profile, to facilitate its coverage by the skin on the medial side of the leg. This also has holes for the fixation screws in the tibial diaphysis (figure 5b 2 ). The channels on each plate fit together, stabilizing the assembly and, at the same time, allowing sliding (figure 5ab).
Figura 5: a1- Placa proximal, vista de frente, b1- Placa distal, vista de frente, a2- Placa proximal, vista de perfil,b2- Placa distal, vista de perfil e ab- encaixe das duas placas, montando o dispositivo.
Figure 5: a1- Proximal plate, front view, b1- Distal plate, front view, a2- Proximal plate, profile view, b2- Distal plate, profile view and ab- fitting of the two plates, assembling the device.

The transferred fibula is interposed between the tibial epiphysis and the distal portion of the tibia.

Subsequently, soft tissue reinsertion, hemostasis, placement of an aspiration drain and closure in layers are performed. After dressing, the limb is placed in an orthosis that was also previously made to measure.

This orthosis is used as an external protective support, until the fibula consolidates and increases its thickness, to be able to support the load (figure 6). This period can vary from 3 to 8 months. 

Figura 6a: cicatriz operatória.
Figure 6a: operative scar.
Figura 6b: ortese confeccionada sob molde.
Figure 6b: mold-made orthosis.

Case 1

                Male patient aged 12 years and 11 months, presenting with osteosarcoma in the proximal region of the right tibia. He underwent wide resection of the tumor, preserving the proximal epiphysis of the tibia. The proximal portion of the fibula with its physis was translated medially to the tibial epiphysis, preserving its blood supply, and osteosynthesis was performed with the sliding fixation device.

After surgery, the limb was kept in an orthosis and weight-bearing was started in the fourth month after surgery, when there were radiographic signs of consolidation. Orthopedic and oncological follow-ups were carried out and the patient returned to his usual activities, with bone consolidation and fibula hypertrophy observed.

 

Case 2

A 2-year-old, 7-month-old male patient with Ewing’s sarcoma in the proximal region of the right tibia underwent resection of the metadiaphyseal segment, compromised by the tumor, preserving the proximal epiphysis of the tibia. The proximal portion of the fibula with its physis was translated medially under the tibial epiphysis maintaining its blood supply and osteosynthesis was performed with the extensible internal fixation device. In this second case, the proximal plate was improved by creating a support to support the remaining tibial plateau, with the aim of increasing stability and avoiding angular deviations (figure 7: a and b). In the distal plate, grooves were made every three mm to facilitate observation of sliding, documenting the growth of the fibula. After surgery, the leg was kept in an orthosis, specially made for the case, and weight-bearing began in the third month after surgery. The patient continued with adjuvant chemotherapy and, in the fourth month, began walking without protection. Bone consolidation and hypertrophy of the fibula can be seen in figure 7: c and d.

Figura 7a: Placa proximal com suporte horizontal, para apoio do planalto da tíbia
Figure 7a: Proximal plate with horizontal support, to support the tibial plateau
Figura 7b: Radiografia do pós operatório imediato.
Figure 7b: Radiograph of the immediate postoperative period.
Figura 7c: Radiografia com 3 meses, mostrando a consolidação tibio-fibular.
Figure 7c: Radiograph at 3 months, showing tibio-fibular consolidation.
Figura 7d: Radiografia com 6 meses, evidenciando a hipertrofia da fíbula.
Figure 7d: Radiograph at 6 months, showing hypertrophy of the fibula.

RESULTS

In the first case, the screws were not locked, which is why valgus deviation occurred, confirmed radiographically, by tilting the screws and clinically (figure 8. abc). This patient underwent the first scanometry of the lower limbs one year after surgery, observing growth of the fibula of 0.75 cm and the reorientation of the screws (figure 8. b).

Figura 8: a- ressonância magnética pré-operatória, b- radiografia pós-operatória 4 meses, evidenciando a inclinação dos parafusos da epífise tibial, c- paciente aos 4 meses, com desvio em valgo do joelho, d- paciente com 1 ano e 2 meses, com o valgo corrigido, e- radiografia pós-operatória 1 ano e 2 meses, com hipertrofia da fíbula, correção da inclinação dos parafusos e crescimento de 0,75 cm e f- radiografia pós-operatória 2 ano e 2 meses, com crescimento de 1,2 cm.
Figure 8: a- pre-operative magnetic resonance imaging, b- 4-month postoperative radiograph, showing the inclination of the tibial epiphysis screws, c- patient at 4 months, with valgus deviation of the knee, d- patient at 1 year old and 2 months, with valgus corrected, e- postoperative radiograph 1 year and 2 months, with fibula hypertrophy, correction of screw inclination and growth of 0.75 cm and radiograph- postoperative radiograph 2 years and 2 months, with growth of 1.2 cm.

The spontaneous correction of this angular deviation, observed clinically, and the horizontalization of the screws, documented radiographically, allows us to confirm the growth of the fibular epiphysis and the sliding of the device. The second scanogram shows growth of 1.2 cm (figure 8. de). The second scanometry was performed at 2 years and 2 months postoperatively, and it was possible to observe growth of 1.2 cm in the transposed fibula segment (figure 8. f).

The patient is a cattle herd, 26 years old, with 14 years of evolution, without recurrence of the disease, with equalized limbs and functionally well (figure 9).

Figure 9: a) paciente com 3 anos e 7 meses de pós-operatório, com carga total, b) com 3 anos e 7 meses, flexão com carga e boa função dos joelhos, e c) radiografia com 3 anos e 7 meses, fibula hipertrofiada, já totalmente tibializada
Figure 9: a) patient at 3 years and 7 months post-surgery, with full weight bearing, b) at 3 years and 7 months, flexion with weight and good knee function, and c) x-ray at 3 years and 7 months, fibula hypertrophied, already fully tibialized

The Patient in Case 2 showed growth of approximately 0.3 cm in the transposed fibula in the first eight months after surgery, a period in which there was orthopedic follow-up.

After this period, the patient in Case 2 showed growth of approximately 0.3 cm in the transposed fibula in the first eight months after surgery, a period in which there was orthopedic follow-up.

The patient died during adjuvant treatment due to chemotherapy complications.

 

DISCUSSION

 

                Reconstruction of bone defects in the proximal region of the tibia represents a challenge for the surgeon, especially when these lesions compromise the growth cartilage.

                Transfemoral amputation is sometimes indicated as the method of choice in young children, due to the difficulty in reconstruction and the discrepancy in the final length of the lower limbs. The use of a prosthetic orthosis, adapted to the amputation stump, can allow early rehabilitation and walking autonomy.

 This method, however, is linked to an important psychological and social impact, due to the mutilation of a large joint, with increased energy expenditure for walking.

Difficulties in adapting to the use of prosthetic orthoses, their high cost and the need to acquire new prostheses as the patient grows are other negative aspects of amputation.

Reconstruction of the proximal segment of the tibia with a non-conventional endoprosthesis is an alternative for these patients. It allows limb preservation and early ambulation. However, high rates of complications related to the method are reported, such as infection, aseptic loosening, mechanical failures, fractures and limitations in physical activities, among others 4,5,16 .

Another important point to be highlighted is the growth of the limbs, both in the operated limb, causing difficulties in adapting the endoprosthesis, and in the opposite limb, causing a discrepancy in length between them. Jeys and collaborators report a series of 661 patients who underwent non-conventional endoprosthesis to treat bone tumors, among which 42% had implant failure within 10 years of follow-up 17 .

Exposure of the endoprosthesis in the proximal third of the tibia and consequent infection are related to the difficulty in covering the implant by the soft tissues in this region.

Wang and collaborators report the use of gastrocnemius and soleus flaps to cover the prosthesis in 11 patients with the aim of avoiding such complications. There were, however, 3 cases of discrepancy between the lower limbs that were treated with contralateral epiphysiodesis 18 .

                The technique of transferring the fibula together with its growth physis presents slower rehabilitation, especially when starting to walk, as it is necessary to wait for bone consolidation between the tibia and the transposed fibula. Subsequently, hypertrophy of the fibula occurs, observed in the cases presented, which makes this segment resistant.

This method represents a biological reconstruction and, therefore, once consolidation and hypertrophy occur, we can consider it as a definitive reconstruction method, performed with a single surgical intervention.

 Preservation of the proximal epiphysis of the tibia allows the articular surface of the knee to be maintained, this represents an advantage of the method when transepiphyseal osteotomy can be performed and is also a necessary condition for its use.

The longitudinal growth capacity of the transposed fibular segment, observed in the cases presented, is also an important factor to be mentioned, as it can avoid or minimize lower limb discrepancy. 

                Bone grafts can also be used to treat this type of injury, especially in cases where the tibial epiphysis can be preserved, which occurs in up to 20% of cases 19 .

However, as it is not a vascularized bone, the graft may not undergo integration and the method is subject to failure, which may result in high rates of fractures or non-union, in addition to not solving the problem of discrepancy 20 . It is difficult to obtain a large quantity of autologous grafts in young children and homologous grafts present greater difficulty in integration, in addition to antigenicity.

 Weitao and collaborators reported the use of allografts in 15 patients with bone sarcomas, with a mean age of 11.75 years (seven to 24) in the distal region of the femur or proximal region of the tibia, where the epiphysis of the bone could be preserved. The results were growth discrepancy in 4 patients, delayed consolidation in 15 patients, graft rejection in 2 cases, infection in 1 case and breakage of the synthesis material with partial reabsorption of the graft in 1 case 7 .

Campanacci and collaborators reported the use of osteoarticular allograft in the reconstruction of osteosarcomas of the distal femur and proximal tibia in which the articular surface was preserved with the tumor. At the end of 5 and 10 years of follow-up, the survival rates of grafts placed in the tibia were only 45% and 20% respectively, with better results in the femoral region 6 .

The advantages of transposition of the fibula over the graft are the fact that it is an osteo-muscular flap, that is, it has vascularization and bone “turn over”, actively participating in consolidation, in addition to maintaining the growth potential of the physis.

As can be seen in figures 7 and 8, the fibula undergoes progressive hypertrophy, a fact that increases its resistance, unlike allografts, which can fail even after years of integration 12,21 .

Distraction osteogenesis with an external fixator can be considered as a reconstruction option. It does not require the use of a graft and makes it possible to fill the bone gap created by tumor resection through bone transport, however, it requires prolonged use of the external fixator and the risk of infection 22 .

Fang and collaborators 8 reported three cases of osteosarcoma in children aged 10 to 14 years, one in the distal region of the femur and two in the proximal region of the tibia, who underwent tumor resection and installation of an Ilizarov apparatus, following the principle of osteogenesis by distension. One of the patients required re-surgical intervention due to loss of bone alignment. Two patients achieved union within eight months, and the fixator was removed. The third patient underwent compression and redistension to achieve bone consolidation. Two cases presented infection along the path of the fixator wires. At the end of two years of follow-up, the lower limb discrepancy was 1.0 to 1.5 cm.

                The use of the ipsilateral fibula for the treatment of tibial injuries, especially in the aftermath of trauma, emerged in 1884, when Hahn described its use for the treatment of tibial pseudarthrosis 11 .

Several techniques have been described and the occurrence of bone consolidation, with fibular hypertrophy, established this method as an alternative in the treatment of tibial defects 9,10,11,12,23,24,25,26,27 .

However, these studies did not use the epiphyseal region of the fibula, limiting its use primarily to diaphyseal injuries of the tibia.

The development of microsurgery has brought a new dimension to the treatment of bone defects.

Taylor and colleagues described the use of the fibula to restore segmental defects in several bones other than the tibia, using microsurgical vascular reconstruction of the fibula 13 .

However, the need for a specialized surgical team, the high cost and the possibility of postoperative arterial or venous thrombosis in microsurgery constitute important disadvantages in relation to techniques that do not require microsurgery  28,29 .

Microsurgery, however, paved the way for the possibility of including the physis in a bone flap, preserving its blood supply and consequently the potential for longitudinal growth of the bone segment 14,30 .

 However, the first reports of growth cartilage transposition presented inconsistent or poor results, with Straub reporting some bone growth in his work in 1929, as fragments of cartilage with or without bone were used in the form of grafts 31-34 .

Based on the description by Pho and collaborators 14 , several studies using a microsurgical technique of transposition of the fibula with the proximal physis and epiphysis showed that the growth potential of the physis could be preserved 35-38 . These studies, however, involve a microsurgical technique for the vascular reconstruction of the fibula, with the disadvantages and complications that the method presents.

The technique presented in this work has the advantage of not requiring any method to reconstruct the vascularization of the fibular segment, with its physis and epiphysis, as the vessels are preserved, as it is a local flap that is positioned under the tibial epiphysis.

We observed bone consolidation, fibular hypertrophy and longitudinal growth of the transplanted segments in the 2 cases presented. In the first case, the patient presented a valgus deformity of the right knee in the immediate postoperative period. With the growth, which occurred in the first year, there was correction of both the valgus deformity and the inclination of the screws, signs of growth of the fibular segment. Over the next fourteen months, the transposed fibular segment grew by another 1.2 cm (Figure 7). This patient, who was a teenager, 12 years old at the time, is now 26 years old, is functionally well and has equalized lower limbs.

In the second case, a child approximately three years old, who could have been ideal for monitoring growth for a longer period, there was a clinical complication that led to death. However, in the first 6 months after surgery, we were able to observe growth of at least 0.3 cm in the transposed fibula segment, which indicates that the growth potential had been preserved.

 

CONCLUSIONS:

 

The extensible internal fixation device stabilizes the reconstruction and allows for growth, through the sliding mechanism between the plates. The proximal fibular segment, transferred using this technique, preserves its blood supply and the growth function of the transplanted physis. We consider that this technique, of transferring the fibula with its growth cartilage, can be used in the reconstruction of injuries that affect the proximal metadiaphyseal segment of the tibia in children, where the tibial epiphysis can be preserved. 

 

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Author: Prof. Dr. Pedro Péricles Ribeiro Baptista

 Orthopedic Oncosurgery at the Dr. Arnaldo Vieira de Carvalho Cancer Institute

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